Researchers said male pythons with implanted radio transmitters were actually used to track breeding females.
With the capture of pythons, researchers are also able to collect data to develop new removal methods.
Big Cypress said the snake sent a new record for the area.
The pythons captured by researchers in Florida typically measure between 6 and 10 feet, researchers said. The Everglades is a vast area with a tropical climate ideal for pythons to hide and thrive, CNN reported.
The python weighed a whopping 140 pounds. Rita Garcia, a spokesperson for the Big Cypress National Preserve, said the eggs were destroyed and the snake was euthanized. The snakes pose significant threats to native wildlife.
To control their population, Florida holds competitions that encourages hunters to remove as many of them as possible.
The inaugural Python Challenge was organised in 2013 by the Florida Fish and Wildlife Conservation Commission and some 1,600 people had registered for this. The searchers found only 68 snakes.
The Resource Management staff would like to thank all of the Preseve divisions that have supported the python program.
Burmese pythons were brought to Florida in the 1970s as pets - however, some were released into the wild, and have since multiplied to great numbers, threatening the survival of local animals.