On Monday, Saudi's foreign ministry recalled its ambassador to Canada for consultations and declared the Canadian ambassador persona non grata, giving him 24 hours to leave.
Local media also reported that Saudi Arabia had been instructed not to transfer its patients to Canada for medical treatment.
Freeland late on Monday also tweeted an official statement saying that Canada would "continue to advocate for human rights and for the fearless women and men who push for these fundamental rights around the world".
The sudden and unexpected dispute bore the hallmarks of Crown Prince Mohammed bin Salman, Saudi Arabia's 32-year-old future leader, whose recent foreign policy exploits include the war in Yemen, the boycott of Qatar and Lebanese Prime Minister Saad Hariri's surprise resignation broadcast during a visit to the kingdom.
"Canada is gravely concerned about additional arrests of civil society and women's rights activists in Saudi Arabia, including Samar Badawi", the tweet read.
Amnesty International noted Ms Badawi has been repeatedly targeted and interrogated by Saudi security forces for her human rights activist.
The clash between Saudi Arabia and Canada is intensifying and has provoked Saudi Airlines to suspend flights to and from Canada starting August 13.
Saudi Arabia's main state wheat buying agency has told grains exporters it will no longer buy Canadian wheat and barley in its global tenders, European traders said on Tuesday, as a diplomatic dispute between the two countries escalates.
"The Kingdom views the Canadian position as an affront to the Kingdom that requires a sharp response to prevent any party from attempting to meddle with Saudi sovereignty", according to a statement by the Saudi foreign ministry. "But I would not deliver Canadian grains to Saudi Arabia now, even on previous contracts", the trader added.
A number of rights campaigners, including award-winning gender rights activist Samar Badawi, have been arrested in the kingdom.
The previous Conservative government also had challenges with Riyadh over human rights.
"We're going to stand with the values that we know are important to Canadians and Saudi Arabia will take the decisions that they take", Morneau said.
His wife, Ensaf Haidar, and three children were granted Canadian citizenship earlier this summer, and live in Quebec's Eastern Townships.
There could be a sentiment among allies that, "Finally the Trudeau government is getting poked back in the eye by the Saudis". At jeopardy are the. thousands of Saudi students in Canada'.