Alexander Yerokhin became the first player to be brought on as a fourth substitute during a World Cup game, replacing Daler Kuzyaev in the 97th minute.
Russian Federation have eliminated 2010 World Cup winners Spain in the tournament's first penalty shootout to secure a spot in the last eight of the competition. Nacho, showing his virtue with some tireless running, earned a free-kick on the right in the 11th minute and Asensio's curler was bundled in for a own goal by Sergey Ignashevich, who was involved in a game of wrestling with Sergio Ramos, the original infant bad.
The 38-year-old was so intent on preventing Spain's Sergio Ramos from getting on the end of Isco's free-kick, he lost sight of the ball before unwittingly turning it into his own net.
The 2018 FIFA World Cup kicked off on June 14 and will continue until July 15, with the matches being played in 11 cities across Russian Federation.
Their elation didn't last long though as Gerard Pique was penalised for handling the ball inside the box. His shot from a tight angle forces Akinfeev into a save!
Spain have not lost a match since Euro 2016, although they have looked far from convincing after the chaos that saw coach Julen Lopetegui sacked before their Federation Internationale de Football Association 2018 first game.
Alba and David Silva then tried to combine, but fine defending from Ilya Kutepov stopped them before Kutepov was booked for a foul on Isco. For the first time in the match a Spanish player gets in behind, with Rodrigo surging forward. Subs - Granat 5, Smolov 6, Cheryshev 6, Erokhin 5.
There was little change to proceedings in the extra period as Spain continued toying with a deep-lying Russian side.
With five minutes to go they were agonisingly close to a victor as Iniesta latched on to a clever touch from Aspas and his low strike from the edge of the area hit the post.
Neither side could find a victor after full-time and the match entered 30 minutes of extra-time.
Sergio Ramos was emotionally drained after Spain's World Cup defeat to Russian Federation and left the pitch at the Luzhniki Stadium in tears just moments after delving into his book of underhand tricks again. But after consulting VAR the referee ruled out a penalty.
Spain's Iago Aspas shot down the middle, and Akinfeev had jumped to his right.